Biodiverzita kultivovatelných prokaryot z Hranické propasti a možnosti uplatnění těchto mikroorganizmů při utváření karbonátových speleotém

Milan Geršl, Marcel Kosina, Ivo Sedláček, Dana Nováková, Fraňo Travěnec

Abstrakt

Hranice Karst is situated in northeastern part of Moravia, Czech Republic, at the contact between two major geological units of the Bohemian Massif and Western Carpathians. This karst developed in combination of meteoric water and hydrothermal dissolution of deformed and tectonically stacked Devonian and Lower Carboniferous limestones. This karst, therefore, exceeds to considerable depths of several hundreds metres. In the 1977, unusually massive organic coatings high in microbial extracellular polymers, occurring together with biofilm structures, were discovered in the vertical caves of low hydrothermally active ‚Hranická propast (Hranice Abyss)‘, particularly on the cave walls in deep underwater environments. These mucilaginous formations may have been classified among ‚snottites‘, mucous-like coatings and stalactites. The present study provides the first insights into both the microbial diversity and morphological/structural variability of these extremophilic formations.

Bibliografická citace

Geršl, M., Kosina, M., Sedláček, I., Nováková, D., & Travěnec, F. (2016). Biodiverzita kultivovatelných prokaryot z Hranické propasti a možnosti uplatnění těchto mikroorganizmů při utváření karbonátových speleotém. Geologické výzkumy na Moravě a ve Slezsku, 14. Získáno z https://journals.muni.cz/gvms/article/view/4835

Klíčová slova

Hranice Karst; Hranice Abyss; biofilm; snottites; isolation; species identification

Plný Text:

Reference

Zobrazit literaturu Skrýt literaturu

Bábek, O. – Novotný, R. (1999): The Hněvotín Limestone neostratotype locality revisited: A conodont biostratigraphy and carbonate microfacies approach, Moravia, Czech Republic. – Acta Univ. Pal. Olom. Fac Rer. Natural., Geologica, 36, 63-68. Olomouc.
Dvořák, J. (1991): Geology of the carbonate evolution of the Devonian and the Lower Carboniferous near Grygov, Přerov, Sobíšky and Hranice (Northern Moravia). – Scripta Geology, 21, 37-62. Brno.
Dvořák, V. (2004): The orientation structural analysis of the Hranice karst limestones. – Geol. Výzk. Mor. Slez. v r. 2003, 11, 42–45. Brno
Haugen, K. – Culver, D. C. (1998): Snapshots of subterranean biodiversity. 1888, 1960 and 1997. – Natn. Speleolog. Soc. Conv., Sewanee, TN, USA, August 3–7, Biology Section Papers, August 4–5, http://www.utexas.edu/tmm/sponsored_sites/biospeleology/nss98abstracts.htm
Häusler J. (1995). Mikrobiologické kultivační metody kontroly jakosti vod – receptář. – Ministerstvo zemědělství České republiky, 13 str., Praha.
Havíř, J. – Bábek, O. – Otava, J. (2004) : Vztah struktur, stratigrafie a krasovění ve Zbrašovských aragonitových jeskyních. – Geol. Výzk Mor. Slez. v r. 2003, 46–50. Brno.
Hladil, J. – Geršl, M. – Strnad, L. – Frána, J. – Langrová, A. – Spišiak, J. (2006): Stratigraphic variation of complex impurities in platform limestones and possible significance of atmospheric dust: a study with emphasis on gamma–ray spectrometry an magnetic susceptibility outcrop logging (Eifelian–Frasnian, Moravia, Czech Republic). – Int. J. Earth Sci., 95, 587–607. Springer, New York.
Jones, D. – Pell, P. A. – Sneath, P. H. A. (1991). Maintenance of bacteria on glass beads at – 60 °C to – 76 °C. – In: Kirsop, B. E. – Doyle, A. (Eds.): Maintenance of Microorganisms and Culture Cells. A Manual of Laboratory Methods. Academic Press. London.
Lee, J. S. – Shin, Y. K. – Yoon, J. H. – Takeuchi, M. – Pyun, Y. R. – Park, Y. H. (2001): Sphingomonas aquatilis sp. nov., Sphingomonas koreensis sp. nov. And Sphingomonas taejonensis sp. nov., yellow-pigmented bacteria isolated from natural mineral. Int. J. Syst. Evol. Microbiology, 51, 1491-1498. Reading, UK.
Morita, R. Y. (1975)“ Psychrophilic Bacteria. – Bacteriol. Rev., 39, 2, 144–167.
Baïda, N. – Yazourh, A. – Singer, E. – Izard, D. (2001). Pseudomonas brenneri sp. nov., a new species isolated from natural mineral waters. Res. Microbiol. 152, 493–502
Northup, D. E. (2001): Geomicrobiology of Caves: A Review. – Geomicrobiology J., 18, 199-222. Taylor & Francis, Philadelphia, PA, USA.
Otava, J. (2005): Polycyclic origin of fossil karst at Hranice Palaeozoic, Czech Republic. – 14th Int. Congr. Speleol., Abstract Book: 121–122, Athens, Greece.
Otava, J., (Ed.) (2004): Vysvětlivky a základní geologická mapa České republiky 1 : 25 000 list 25-123 Hranice. – MS, Archiv Česká geologická služba. Praha-Brno.
Popovic, T. – Bopp, C. A. – Olsvik, Ø. – Kiehlbauch, J. A. (1993): Ribotyping in molecular epidemiology. – In: Persing, D. H. – Smith, T. F. – Tenover, F. C. – White T. J. (Eds.): Diagnostic molecular microbiology: principles and applications. 573–583. ASM Press, Washington, USA.
Pot, B. – Vandamme, P. – Kersters, K. (1994): Analysis of electrophoretic whole-organism protein fingerprints. In: Goodfellow, M., O’Donnell, A.G. (Eds.): Modern microbiological methods. Chemical methods in Prokaryotic systematics. John Wiley and Sons, 493-521. Chichester, UK.
Travěnec, F. (1977): Sabbath diving logbook. – MS, Archiv F. Travěnec, Olomouc.
Travěnec, F. (1982): 110 m s héliovzduchovou směsí v Hranické Propasti. – Stalagmit, 4, 1–2, 6–7. Čes. speleolog. spol. Praha.

Travěnec, F. (2001): Soplíky v Hranické Propasti. Předběžná zpráva. – MS, Archiv ZO ČSS 6-23 Aragonit, Hranice, 6 str. Olomouc.
Verhille, S. – Baida, N. – Dabboussi, F. – Izard, D. – Leclerc, H. (1999): Taxonomic study of bacteria isolated from natural mineral waters: proposal of Pseudomonas jessenii sp. nov. and Pseudomonas mandelii sp. – Syst. Appl. Microbiol., 22(1), 45–58. G. Fischer Verlag, Stuttgart.
Quevedo-Sarmiento, J. – Ramos-Cormenzana, A. – Gonzalez-Lopez, J. (1986): Isolation and characterization of aerobic heterotrophic bacteria from natural spring waters in the Lanjaron area (Spain). – J. Appl. Bacteriol. 61, 4, 365–372. Blackwell Publishing, Oxford.
Svec, P. – Stegnerova, H. – Durnova, E. – Sedlacek, I. (2004): Characterization of esculin-positive Pseudomonas fluorescens strains isolated from an underground brook. Folia Microbiol., 49(6), 725-730. Praha.