Blood oxidative stress markers in the 32nd Bulgarian Antarctic expedition participants
Vol.15,No.1(2025)
Antarctic expeditions are associated with significant stress for participants related to long journeys, work in adverse environmental conditions, and social contact changes resulting in oxidative stress (OS) at the molecular level. This study aimed to evaluate the blood serum OS of the participants in the 32nd Bulgarian Antarctic Expedition (2023-2024) and related trace elements iron, copper, zinc, and selenium concentrations, lipid profile, and blood parameters. Blood parameters and trace element concentrations were measured in a clinical laboratory. OS indicators were measured spectrophotometrically using kits. The results showed decreased antioxidant enzyme activities and increased glutathione concentration after the expedition in the blood serum from both females and males. No statistical differences in the values of OS parameters between males and females were observed. However, the mean serum total antioxidant capacity after the expedition in males was two times higher than in females. In males, a statistically significant lower erythrocyte sedimentation rate (p<0.05), ferritin (p<0.001), and zinc (p<0.01) levels, catalase (p<0.001) and superoxide dismutase (p<0.05) activities were observed after the expedition. No correlation between enzyme activities and the studied trace element concentrations was found. In conclusion, it can be assumed that the expeditioners’ OS was higher before the expedition, likely because of psychological and physical stress related to overcoming preparation and logistics problems. After the expedition, OS has subsided. In order to maintain good health and increase physical fitness, it could be recommended that polar expeditioners receive dietary supplements, micronutrients, and vitamins in the setting of rational nutrition, especially before an expedition.
Antarctics; antioxidants; human; lipid peroxidation; serum trace elements; Livingston Island; Kliment Ohridski Station; Austral summer
Bui, V. Q., Stein, A. D., Di Girolamo, A. M., Ramakrishnan, U., Flores-Ayala, R. C., Ramirez-Z, M., Grant F. K., Villalpando, S. and Martorell, R. (2012): Associations between serum C-reactive protein and serum zinc, ferritin, and copper in Guatemalan school children. Biological Trace Element Research, 148(2): 154-160. doi: 10.1007/s12011-012-9358-0
Chang, C. J., Brady, D. C. (2023): Capturing copper to inhibit inflammation. Nature Chemical Biology, 19: 937-939. doi: 10.1038/s41589-023-01383-6
Davis, C. E., Williams, D. H., Oganov, R. G., Tao, S. C., Rywik, S. L., Stein, Y. and Little, J. A. (1996): Sex difference in high density lipoprotein cholesterol in six countries. American Journal of Epidemiology, 143(11): 1100-1106. doi: 10.1093/oxfordjournals.aje.a008686
Domuschieva-Rogleva, G., Iancheva, T. (2017): Coping strategies for the participants in the Antarctic expedition. Journal of Sport Psychology, 26(4): 45-50.
Domuschieva-Rogleva, G., Iancheva, T. and Shopov, A. (2017): Dynamics of anxiety and perceived stress among the participants in the XXV antarctic expedition. Journal of Applied Sports Sciences, 2: 31-41. doi: 10.37393/jass.2017.02.4
Emokpae, M. A., Fatimehin, E. B. (2020): Copper-to-Zinc ratio as an inflammatory marker in patients with sickle cell disease. Sci, 2(4): 89. doi: 10.3390/sci2040089
Fernández-Lázaro, D., Domínguez-Ortega, C., Busto, N., Santamaría-Peláez, M., Roche, E., Gutiérez-Abejón, E. and Mielgo-Ayuso, J. (2023): Influence of N-Acetylcysteine Supplementation on Physical Performance and Laboratory Biomarkers in Adult Males: A Systematic Review of Controlled Trials. Nutrients, 15(11): 2463. doi: 10.3390/nu15112463
Halliwell, B., Gutteridge, J. M. C. (1999): Free Radicals in Biology and Medicine. Oxford University Press, New York, 897 p.
Halsey, L. G., Stroud, M. A. (2012): 100 years since Scott reached the pole: A century of learning about the physiological demands of Antarctica. Physiological Reviews, 92(2): 521-536. doi: 10.1152/physrev.00031.2011
Han, L. L., Wang, Y. X., Li, J., Zhang, X. L., Bian, C., Wang H., Du, S. and Suo, L. N. (2014): Gender differences in associations of serum ferritin and diabetes, metabolic syndrome, and obesity in the China Health and Nutrition Survey. Molecular Nutrition & Food Research, 58(11): 2189-2195. doi: 10.1002/mnfr.201400088
Iancheva, T., Boyanov, D. and Panayotov, N. (2023): Stress factors and preferred coping strategies of participants in XXXI Bulgarian Antarctic Expedition. Journal of Applied Sports Sciences, 1: 47-59. doi: 10.53656/str2023-5s-1-nee
Iancheva, T., Domuschieva–Rogleva, G. and Kuleva, M. (2018): Sensation seeking and stress coping strategies of participants in military missions in Afghanistan and Antarctic expeditions. Journal of Applied Sports Sciences, 1: 53-63. doi: 10.37393/jass.2018.01.7
Iuliano, S., Ayto, N. J. (2015): Dietary intakes of expeditioners during prolonged sunlight deprivation in polar environments do not support bone health. International Journal of Circumpolar Health, 74: 27965. doi: 10.3402/ijch.v74.27965
Johnsen, B. H., Gjeldnes, R. (2023): Back to the basics of polar expeditions: personality hardiness, fear, and nutrition in polar environments. Safety in Extreme Environments, 5: 47-58. doi: 10.1007/s42797-023-00068-6
Kell, D. B., Pretorius, E. (2014): Serum ferritin is an important inflammatory disease marker, as it is mainly a leakage product from damaged cells. Metallomics: Integrated Biometal Science, 6(4): 748-773. doi: 10.1039/c3mt00347g
Ledwozyw, A., Michalak, J., Stepień, A. and Kadziołka, A. (1986): The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis. International Journal of Clinical Chemistry, 155(3): 275-283. doi: 10.1016/0009-8981(86)90247-0
Loeper, J., Ement, J., Goy, J., Bedu, O. and Leoper, J. (1983): Lipid peroxidation during human atherosclerosis. IRCS Medical Science, 11: 1035-1043.
Mäkinen, T. M. (2007): Human cold exposure, adaptation, and performance in high latitude environments. American Journal of Human Biology, 19(2): 155-164. doi: 10.1002/ajhb.20627
Margaritelis, N., Theodorou, A., Paschalis, V., Veskoukis, A., Dipla, K., Zafeiridis, A., Panayiotou, G., Vrabas, I., Kyparos, A. and Nikolaidis, M. (2018): Adaptations to endurance training depend on exercise-induced oxidative stress: Exploiting redox interindividual variability. Acta Physiologica (Oxford, England), 222. doi: 10.1111/apha.12898
Marseglia, L., Manti, S., D'angelo, G., Nicotera, A., Parisi, E., Di Rosa, G., Gitto, E. and Arrigo, T. (2014): Oxidative stress in obesity: a critical component in human diseases. International Journal of Molecular Sciences, 16(1): 378-400. doi: 10.3390/ijms16010378
Matuz-Mares, D., Riveros-Rosas, H., Vilchis-Landeros, M. M. and Vázquez-Meza, H. (2021): Glutathione participation in the prevention of cardiovascular diseases. Antioxidants (Basel, Switzerland), 10(8): 1220. doi: 10.3390/antiox10081220
Moiseyenko, Y., Sukhorukov, V., Pyshnov, G., Mankovska, I., Rozova, E. V., Miroshnychenko, O., Kovalevska, O., Madjar, S. A., Bubnov, R., Gorbach, A., Danylenko, K. and Moiseyenko, O. (2016): Antarctica challenges the new horizons in predictive, preventive, personalized medicine: Preliminary results and attractive hypotheses for multi-disciplinary prospective studies in the Ukrainian "Akademik Vernadsky" station. EPMA Journal, 7(1): 11. doi: 10.1186/s13167-016-0060-8
Morón, U. M., Castilla-Cortázar, I. (2012): Protection against oxidative stress and “IGF-I Deficiency Conditions”. Antioxidant Enzyme, IntechOpen. doi: 10.5772/51047
Nan, Y., Bai, Y. (2022): Sex-based differences in the association between serum copper and kidney function: Evidence from NHANES 2011–2016. International Journal of Environmental Research and Public Health, 19(21): 14086. doi: 10.3390/ijerph192114086
Oksa, J., Kaikkonen, H., Sorvisto, P., Vaappo, M., Martikkala, V. and Rintamäki, H. (2004): Changes in maximal cardiorespiratory capacity and submaximal strain while exercising in cold. Journal of Thermal Biology, 29(7–8): 815-818. doi: 10.1016/j.jtherbio.2004.08.063
Palinkas, L.A., Suedfeld, P. (2008): Psychological effects of polar expeditions. Lancet (London, England), 371(9607): 153-163. doi: 10.1016/S0140-6736(07)61056-3
Parent, A. A., Martin, D., Morales, S., Boucher, J. and Comtois, A. S. (2018): XPAntarctik Expedition: The effect of a month-long expedition in Antarctica on physiological performance. Journal of Human Performance in Extreme Environments, 14(1): 10. doi: 10.7771/2327-2937.1093
Pérez, L. M., Hooshmand, B., Mangialasche, F., Mecocci, P., Smith, A. D., Refsum, H., Inzitari, M., Fratiglioni, L., Rizzuto, D. and Calderón-Larrañaga, A. (2020): Glutathione serum levels and rate of multimorbidity development in older adults. The Journal of Gerontology. Series A, Biological Sciences and Medical Sciences, 75(6): 1089-1094. doi: 10.1093/gerona/glz101
Premkumar, M., Sable, T. (2012): Obesity, dyslipidemia and cholesterol gallstone disease during one year of Antarctic residence. Rural and Remote Health, 12: 2186.
Quinn, J. F., Harris, C., Kaye, J. A., Lind, B., Carter, R., Anekonda, T. and Ralle, M. (2011): Gender effects on plasma and brain copper. International Journal of Alzheimer's disease, 150916. doi: 10.4061/2011/150916
Rozova, E. V., Moiseyenko, Y. V. (2013): Long stay effects in the Antarctic on ultrastructure and platelet function in people with load hypoxic. Ukrainian Antarctic Journal, 12: 307-314. doi: 10.33275/1727-7485.12.2013.273
Russo, G. T., Giandalia, A., Romeo, E. L. and Cucinotta, D. (2015): Gender differences in lipoprotein metabolism. The Italian Journal of Gender-Specific Medicine, 1(2): 58-65. doi: 10.1723/2188.23640
Salekeen, R., Haider, A. N., Akhter, F., Billah, M. M., Islam, M. E. and Didarul Islam, K. M. (2022): Lipid oxidation in pathophysiology of atherosclerosis: Current understanding and therapeutic strategies. International Journal of Cardiology. Cardiovascular Risk and Prevention, 14: 200143. doi: 10.1016/j.ijcrp.2022.200143
Selye, H. (1950): Stress and the general adaptation syndrome. British Medical Journal, 1(4667): 1383-1392. doi: 10.1136/bmj.1.4667.1383
Song, X., Wang, W., Li, Z. and Zhang, D. (2018): Association between serum copper and serum lipids in adults. Annals of Nutrition & Metabolism, 73(4): 282-289. doi: 10.1159/000494032
Spinelli, E., Werner Junior, J. (2022): Human adaptative behavior to Antarctic conditions: A review of physiological aspects. WIREs Mechanisms of Disease, 14(5): e1556. doi: 10.1002/wsbm.1556
Stocks, J. M., Taylor, N. A., Tipton, M. J. and Greenleaf, J. E. (2004): Human physiological responses to cold exposure. Aviation, Space, and Environmental Medicine, 75(5): 444-457.
Tan, M., Yin, Y., Ma, X., Zhang, J., Pan, W., Tan, M., Zhao, Y., Yang, T., Jiang, T. and Li, H. (2023): Glutathione system enhancement for cardiac protection: Pharmacological options against oxidative stress and ferroptosis. Cell Death and Disease, 14: 131. doi: 10.1038/s41419-023-05645-y
Tenório, M. C. D. S., Graciliano, N. G., Moura, F. A., Oliveira, A. C. M. and Goulart, M. O. F. (2021): N-Acetylcysteine (NAC): Impacts on human health. Antioxidants (Basel, Switzerland), 10(6): 967. doi: 10.3390/antiox10060967
Wada, A., Sakura, S., Kobayashi, H., Yanagihara, N. and Izumi, F. (1983): Suppression by phospholipase A2 inhibitors of secretion of catecholamines from isolated adrenal medullary cells by suppression of cellular calcium uptake. Biochemical Pharmacology, 32(7): 1175-1178. doi: 10.1016/0006-2952(83)90267-8
Yuan, Y., Long, P., Liu, K., Xiao, Y., He, S., Li, J., Mo, T., Liu, Y., Yu, Y., Wang, H., Zhou, L., Liu, X., Yang, H., Li, X., Min, X., Zhang, C., Zhang, X., Pan, A., He, M., Hu, F. B. and Wu, T. (2020): Multiple plasma metals, genetic risk and serum C-reactive protein: A metal-metal and gene-metal interaction study. Redox Biology, 29: 101404. doi: 10.1016/j.redox.2019.101404
Žákovská, А., Zezulová, О. (2019): Changes in haematological parameters during a summer expedition in Antarctica. Czech Polar Reports, 9(1): 107-113. doi: 10.5817/CPR2019-1-9
Žákovská, А., Zezulová, О. (2016): Some of the metabolic changes in expedition members caused by diet and activities performed during a stay at the Czech Antarctic base. Czech Polar Reports, 6(1): 13-20. doi: 10.5817/CPR2016-1-2
Web sources / Other sources
[1] Meteoblue, Switzerland
https://www.meteoblue.com/en/weather/historyclimate/weatherarchive/st.-kliment-ohridski-base_antarctica_6620786
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