Photoinhibition of primary   		photosynthetic processes  in  		 hydrated Polytrichum  commune:  Analysis  		 of  non-photochemical  		 quenching affecting species resistance

Gabriella Nora Maria Giudici

doi:10.5817/CPR2019-2-14

Photoinhibition of primary photosynthetic processes in hydrated Polytrichum commune: Analysis of non-photochemical quenching affecting species resistance

Vol.9,No.2(2019)

Gabriella Nora Maria Giudici

https://doi.org/10.5817/CPR2019-2-14

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Abstract

A moss from the alpine environment of the Jeseníky mountains, Polytrichum commune, was studied under lab-induced light stress to analyse photoinhibition (PI) stress response; three PI doses were used: PAR 1500 µmol m^-2 s^-1 for 60 min., 1200 µmol m^-2 s^-1 for 60 min. and 1200 µmol m^-2 s^-1 for 30 min.; in the last one the added component of slight desiccation stress was added. Chlorophyll fluorescence parameters were plotted as time series, immediately before and after the PI treatments, then every 20 minutes for three hours (recovery period). F_V/F_M, Φ_PSII and NPQ parameters and quenching components were analysed. Decreasing courses and final values of F_V/F_M and Φ_PSIIparameters along with increased values of NPQ clearly indicated PI stress response, although not very severe. Quenching parameters analysis showed a dominant role played by xanthophyll pigments along with changes in PS II in the non-photochemical energy quenching. Dehydration contributed additional value to NPQ. All these factors are consistent with the adaptation of the species to harsh conditions of alpine environments.

Keywords:
NPQ quenching; Polytrichum; chlorophyll fluorescence; photoinhibition; lamellae; alpine environment

References

Alboresi, A., Gerotto, C., Giacometti, G. M., Bassi, R. and Morosinotto, T. (2010): Physcomitrella patens mutants afected on heat dissipation clarify the evolution of photoprotection mechanisms upon land colonization. Proceedings of National Academy of Science USA, 107: 11128-11133. Csintalan, Z., Proctor, M. C. F. and Tuba, Z. (1999): Chlorophyll fluorescence during drying and rehydration in the mosses Rhytidiadelphus loreus (Hedw.) Warnst., Anomodon viticulosus (Hedw.) Hook. & Tayl. and Grimmia pulvinata (Hedw.) Sm. Annals of Botany, 84: 235-244. Dikaios, I., Schiphorst, Ch., Dall’Osto, L., Alboresi, A., Bassi, R. and Pinnola, A. (2019): Functional analysis of LHCSR1, a protein catalyzing NPQ in mosses, by heterologous expression in Arabidopsis thaliana. Photosynthesis Research, 142: 249-264. Gerotto, C., Alboresi, A., Giacometti, G. M., Bassi, R. and Morosinotto, T. (2012): Coexistence of plant and algal energy dissipation mechanisms in the moss Physcomitrella patens. New Phytologist, 196: 763-773. Hájek, T., Tuittila, E.-S., Ilomets, M. and Laiho, R. (2009): Light responses of mire mosses: a key to survival after water-level drawdown? Oikos, 118: 240-250. Heber, U. (2008): Photoprotection of green plants: A mechanism of ultra-fast thermal energy dissipation in desiccated lichens. Planta, 228: 641-650. Heber, U. (2011): Lichens and Bryophytes: Light Stress and Photoinhibition in Desiccation/ Rehydration Cycles – Mechanisms of Photoprotection. In: U. Lüttge, E. Beck, D. Bartels (eds.): Plant Desiccation Tolerance. Springer, 386 p. Heber, U., Bilger, W. and Shuvalov, V. A. (2006): Thermal energy dissipation in reaction centres and in the antenna of photosystem II protects desiccated poikilohydric mosses against photo-oxidation. Journal of Experimental Botany, 57(12): 2993-3006. doi: 10.1093/jxb/erl058 Heber, U., Azarkovich, M. and Shuvalov, V. A. (2007): Activation of mechanisms of photoprotection by desiccation and by light: Poikilohydric photoautotrophs. Journal of Experimental Botany, 58: 2745-2759. Heber, U., Bilger, W., Türk, R. and Lange, O. L. (2009) Photoprotection of reaction centres in photosynthetic organisms: mechanisms of thermal energy dissipation in desiccated thalli of the lichen Lobaria pulmonaria. New Phytologist, doi: 10.1111/j.1469-8137.2009.03064.x Jägerbrand, A. K.,Kudo, G. (2014): Short-term responses in maximum quantum yield of PSII (Fv/Fm) to ex situ temperature treatment of populations of bryophytes originating from different sites in Hokkaido, Northern Japan. Plants (Basel). 2016 Jun; 5(2): 22. doi: 10.3390/ plants5020022. Ljubešic, N., Wrischer, M., Prebeg, T. and Devidé, Z. (2005): Structural changes of lamellar cells in leaves of the moss Polytrichum formosum Hedw. during winter freezing and thawing processes. Acta Botanica Croatica, 64: 219-226. Lovelock, C. E., Osmond, C. and Seppelt, R. D. (1995a): Photoinhibition in the Antarctic moss Grimmia antarctici card when exposed to cycles of freezing and thawing. Plant Cell and Environment, 18: 1395-1402. Lovelock, C. E., Jackson, A. E., Melick, D. R. and Seppelt, R. D. (1995b): Reversible photoinhibition in Antarctic moss during freezing and thawing. Plant Physiology, 109: 955-961. Marschall, M., Proctor, M. C. F. (2004): Are bryophytes shade plants? Photosynthetic light responses and proportions of chlorophyll a, chlorophyll b and total carotenoids. Annals of Botany, 94: 593-603. Murray, K. J., Tenhunen, J. D. and Nowak, R. S. (1993): Photoinhibition as a control on photosynthesis and production of Sphagnum mosses. Oecologia, 96: 200-207. Nabe, H., Funabiki, R., Kashino, Y., Koike, H. and Satoh, K. (2007): Responses to desiccation stress in bryophytes and an important role of dithiothreitol-insensitive non-photochemical quenching against photoinhibition in dehydrated states. Plant and Cell Physiology, 48(11): 1548-1557. Post, A. (1990): Photoprotective pigment as an adaptive strategy in the Antarctic moss Ceratodon purpureus. Polar Biology, 10: 241-245. Pinnola, A., Ghin, L., Gecchele, E., Merlin, M., Alboresi, A., Avesani, L., Pezzotti, M., Capaldi, S., Cazzaniga, S. and Bassi, R. (2015): Heterologous expression of moss Light-harvesting Complex Stress-related 1 (LHCSR1), the chlorophyll a-xanthophyll pigment-protein complex catalyzing non-photochemical quenching, in Nicotiana sp. The Journal of Biological Chemistry, 290: 24340-24354. Proctor, M. C. F., Ligrone, R. and Duckett, J. G. (2007): Desiccation tolerance in the moss Polytrichum formosum: Physiological and fine structural changes during desiccation and recovery. Annals of Botany, 99: 75-93. Robinson, S., Waterman, M. (2014): Sunsafe bryophytes: Photoprotection from excess and damaging solar radiation. In: D. T. Hanson, S. K. Rice: Photosynthesis in bryophytes and early land plants. Springer, pp. 113-130. Stella, G. R. (2016): Light stress and photoprotection in green algae, mosses and diatoms. Ph.D. thesis, University of Verona and University Pierre et Marie Curie, 144 p. Thomas, R. J., Ryder, S. H., Gardner, M. I., Sheettz, J. P. and Nichipor, S. D. (1996): Photosynthetic functions of leaf lamellae in Polytrichum commune. The Bryologist, 99: 11. Veerman, J., Vasilev, S., Paton, G. D., Ramanauskas, J. and Bruce, D. (2007): Photoprotection in the lichen Parmelia sulcata: The origins of desiccation induced fluorescence quenching. Plant Physiology, 145: 997-1005. Yamakawa, H., Fukushima, Y., Itoh, S. and Heber, U. (2012): Three different mechanisms of energy dissipation of a desiccation-tolerant moss serve one common purpose: To protect reaction centres against photo-oxidation. Journal of Experimental Botany,63: 3765-3775. Yamakawa, H., Itoh, S. (2013): Dissipation of excess excitation energy by drought-induced nonphotochemical quenching in two species of drought-tolerant moss: desiccation-induced acceleration of photosystem II fluorescence decay. Biochemistry, 52: 4451-4459.

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