Fungal species associated with fruit and vegetables transported to the J.G. Mendel station and the influence of UV-C treatment on their fungal community
Vol.9,No.1(2019)
The aim of this study was to investigate the fungal community associated with fruits and vegetables transported into the Antarctic region and observe qualitative changes of their surface mycobiota after UV-C treatment. This measure is used to prevent the post-harvest diseases of stored fruits and vegetables and reduce the risk of introducing non-native species to the Antarctic environment. In total, 82 strains of filamentous fungi were isolated from the surfaces of 64 pieces of fresh fruits and vegetables before and after their UV-C treatment. They were assigned to the genera Penicillium, Fusarium, Mucor, Cladosporium, and Acremonium. After the UV-C treatment of the examined fruits and vegetables, spores of the genera Fusarium, Cladosporium and Acremonium were not detected, while spores of the genera Penicillium and Mucor were more resistant and stayed viable after the treatment. Penicillium strains prevailed in the examined samples. Their introduction to the Antarctic environment could represent a potential risk for endemic autochthonous organisms.
non-native; fungi; fresh food; Antarctic region; UV-C treatment; post-harvest diseases
Adesemoye, A., Eskalen, A., Faber, B., Bender, G., O’Connell, N., Kallsen, C. and Shea, T. (2011): Current knowledge on Fusarium dry rot of citrus. Citrograph, November/December 2011: 29-33.
Alborés, S., Sanguiedo, P., Held, B. H., Cerdeiras, M. P. and Blanchette, R. A. (2018): Biodiversity and antimicrobial activity of Antarctic fungi from the Fildes Peninsula, King George Island. Sydowia, 70: 185-191.
Arenz, B. E., Held, B. W., Jurgens, J. A., Farrell, R. L. and Blanchette, R. A. (2006): Fungal diversity in soils and historic wood from the Ross Sea region of Antarctica. Soil Biology and Biochemistry, 38: 3057-3064.
Augustyniuk-Kram, A., Chwedorzewska, K. J., Korczak-Abshire, M., Olech, M. and Lityńska-Zając, M. (2013): An analysis of fungal propagules transported to the Henryk Arctowski Station. Polish Polar Research, 34: 269-278.
Begum, M., Hocking, A. D. and Miskelly, D. (2009): Inactivation of food spoilage fungi by ultra violet (UVC) irradiation. International Journal of Food Microbiology, 129: 74-77.
Bridge, P. D., Spooner, B. M. and Roberts, P. J. (Version 2.3.3; January 2010): Non-lichenized fungi from the Antarctic region. Available at https://legacy.bas.ac.uk/bas_research/data/access/ fungi/ (accessed 18. 2. 2019).
Chwedorzewska, K. J., Korczak-Abshire, M., Olech, M., Lityńska-Zając, M. and Augustyniuk-Kram, A. (2013): Alien invertebrates transported accidentally to the Polish Antarctic Station in cargo and on fresh foods. Polish Polar Research, 34: 55-66.
Coates, L. M., Johnson, G. I. and Dale, M. (1997): Postharvest pathology of fruit and vegetables. In: J. Brown and H. Ogle (eds.): Plant Pathogens and Plant Diseases. Rockvale Publications Editors, Armidale, Australia, pp. 533-547.
Cowan, D. A., Chown, S. L., Convey, P., Tuffin, M., Hughes, K. A., Pointings, S. and Vincent, W. F. (2011): Non-indigenous microorganisms in the Antarctic: assessing the risks. Trends in Microbiology, 19: 540-548.
Czarnecki, B., Bialasiewicz, D. (1987): Fungi as a component of the aerosphere in the H. Arctowski polar station and its vicinity (King George Island, South Shetland Islands). Polish Polar Research, 8: 153-158.
Domsch, K. H., Gams, W. and Anderson, T. H. (1980): Compendium of soil fungi. Academic Press, London, United Kingdom, 860 p.
Frisvad, J. C., Samson, R. A. (2004): Polyphasic taxonomy of Penicillium subgenus Penicillium. A guide to identification of food and air-borne terverticillate Penicillia and their mycotoxins. Studies in Mycology, 49: 1-173.
Galera H., Chwedorzewska, K. J., Korczak-Abshire, M. and Wódkiewicz, M. (2018): What affects the probability of biological invasions in Antarctica? Using an expanded conceptual framework to anticipate the risk of alien species expansion. Biodiversity and Conservation, 27: 1789-1809.
Gonçalves, V. N., Campos, L. S., Melo, I. S., Pellizari, V. H., Rosa, C. A. and Rosa, L. H. (2013): Penicillium solitum: a mesophilic, psychrotolerant fungus present in marine sediments from Antarctica. Polar Biology, 36: 1823-1831.
Gündüz, G. T., Pazir, F. (2012): Inactivation of Penicillium digitatum and Penicillium italicum under in vitro and in vivo conditions by using UV-C light. Journal of Food Protection, 76: 1761–1766.
Hernández-Montiel, L. G., Ochoa, J. L., Troyo-Diéguez, E. and Larralde-Corona, C. P. (2010): Biocontrol of postharvest blue mold (Penicillium italicum Wehmer) on Mexican lime by marine and citrus Debaryomyces hansenii isolates. Postharvest Biology and Technology, 56: 181-187.
Hill, J. L, Hocking, A. D. and Whitfield, F. B. (1995): The role of fungi in the production of chloroanisoles in general purpose freight containers. Food Chemistry, 54: 161-166.
Hughes, K. A., Convey, P. (2010): The protection of Antarctic terrestrial ecosystems from inter- and intra-continental transfer of non-indigenous species by human activities: a review of current systems and practices. Global Environmental Change, 20: 96-112.
Hughes, K.A., Convey, P. (2012): Determining the native/non-native status of newly discovered terrestrial and freshwater species in Antarctica - current knowledge, methodology and management action. Journal of Environmental Management, 93: 52-66.
Hughes, K. A., Cowan, D. A. and Wilmotte, A. (2015): Protection of Antarctic microbial communities - ‘out of sight, out of mind’. Frontiers in Microbiology, 6: 1-6.
Hughes K. A., Lee, J. E., Tsujimoto, M., Imura, S., Bergstrom, D. M.,Ware, C., Lebouvier, M. L., Huiskes, A. H. L., Gremmen, N. J. M., Frenot, Y., Bridge, P. D. and Chown, S. L. (2011): Food for thought: Risks of non-native species transfer to the Antarctic region with fresh produce. Biological Conservation, 144: 1682-1689.
Hughes K. A., Misiak, M., Yogbaanu, U. and Newsham, K. K. (2018) Importation of psychrotolerant fungi to Antarctica associated with wooden cargo packaging. Antarctic Science, 30: 298-305.
Huiskes, A. H. L., Gremmen, N. J. M., Bergstrom, D. M., Frenot, Y., Hughes, K. A., Imura, S., Kiefer, K., Lebouvier, M., Lee, J. E., Tsujimoto, M., Ware, C., Van de Vijver, B. and Chown, S. L. (2014): Aliens in Antarctica: assessing transfer of plant propagules by human visitors to reduce invasion risk. Biological Conservation, 171: 278-284.
Kostadinova, N., Krumova, E., Tosi, S., Pashova, S. and Angelova, M. (2009): Isolation and identification of filamentous fungi from Island Livingston, Antarctica. Biotechnology and Biotechnological Equipment, 23: 267-270.
Laichmanová, M., Selbmann, L. and Barták, M. (2009): Diversity of microfungi from James Ross Island, Antarctica. In: M. Barták, J. Hájek, P. Váczi (eds.): Structure and Function of Antarctic Terrestrial Ecosystems. Book of Abstracts and Contributed Papers. Conference, Brno, October 22-23, 2009. Masaryk Univerzity, Brno, Czech Republic, pp. 10-13.
Leslie, J. F., Summerell, B. A. (2006): The Fusarium laboratory manual. Blackwell Publishing, Iowa, USA, 388 p.
Lityńska-Zając, M., Chwedorzewska, K. J., Olech, M., Korczakabshire, M. and Augustyniuk-Kram, A. (2012): Diaspores and phytoremains accidentally transported to the Antarctic Station during three expeditions. Biodiversity and Conservation, 21: 3411-3421.
Lugauskas, A., Repeckiene, J. and Novosinskas, H. (2005): Micromycetes, producers of toxins, detected on stored vegetables. Annals of Agricultural and Environmental Medicine, 12: 253-260.
Molina-Montenegro, M. A., Pertierra, L. R., Razeto-Barry, P., Díaz, J., Finot, V. L. and Torres-Díaz, C. (2015): A recolonization record of the invasive Poa annua in Paradise Bay, Antarctic Peninsula: modeling of the potential spreading risk. Polar Biology, 38: 1091-1096.
Onofri, S., Zucconi, L. and Tosi, S. (2007): Continental Antarctic Fungi. NHBS - IHWVerlag, Eching, Germany, 247 p.
Osyczka, P., Mleczko, P., Karasinski, D. and Chlebicki, A. (2012): Timber transported to Antarctica: a potential and undesirable carrier for alien fungi and insects. Biological Invasions, 14: 15-20.
Pitt, J. I., Hocking, D. (1997): Fungi and Food Spoilage. 2nd ed. Blackie Academic and Professional, London, United Kingdom, 593 p.
Rodov, V., Yehoshua, S. B., Kim, J. J., Shapiro, B. and Ittah, Y. (1992): Ultraviolet illumination induces scoparone production in kumquat and orange fruit and improves decay resistance. Journal of the American Society for Horticultural Science, 117: 788-792.
Rosa, L. H., Vieira, M. L. A., Santiago, I. F. and Rosa, C. A. (2010): Endophytic fungi community associated with the dicotyledonous plant Colobanthus quitensis (Kunth) Bartl. (Caryophyllaceae) in Antarctica. FEMS Microbiology Ecology, 73: 178-189.
Ruisi, S., Barreca, D., Selbmann, L., Zucconi, L. and Onofri, S. (2007): Fungi in Antarctica. Reviews in Environmental Science and Biotechnology, 6: 127-141.
Uyar, G. E. Ö., Uyar, B. (2018, in press): Effects of ethanol and ultraviolet-c treatments on inactivation of Rhizopus oryzae spores which cause postharvest rot. Food Science and Technology, DOI: https://doi.org/10.1590/fst.04618.
Valdez, J. G., Makuch, M. A., Ordovini, A. F., Frisvad, J. C., Overy, D. P., Masuelli, R. W. and Piccolo, R. J. (2009): Identification, pathogenicity and distribution of Penicillium spp. isolated from garlic in two regions in Argentina. Plant Pathology, 58: 352-361.
Valero, A., Begum, M., Leong, S. L., Hocking, A. D., Ramos, J., Sanchis, V. and Marín, S. (2007): Effect of germicidal UVC light on fungi isolated from grapes and raisins. Letters in Applied Microbiology, 45: 238-243.
Zycha, H., Siepmann, R. and Linnemann, G. (1969): Mucorales, eine Beschreibung aller Gattungen und Arten dieser Pilzgruppe. Cramer, Lehre, Germany, 355 p. (In German).
Web sources / Other sources
[1] COMNAP and SCAR (2010): Checklists for supply chain managers of national Antarctic programmes for the reduction in risk of transfer of non-native species. Available at https://www.comnap.aq/SitePages/checklists.aspx.
[2] Committee for Environmental Protection, CEP (2017): Non-native species manual. Secretariat of the Antarctic Treaty, Buenos Aires, Argentina, 41 p.
[3] Czech Collection of Microorganisms (CCM), Masaryk University, Brno, http://www.sci.muni.cz/ccm/,
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Copyright © 2020 Czech Polar Reports