Antarctic strain of green filamentous alga Zygnema sp. shows a high resistance to photoinhibition under simulated polar conditions
Vol.5,No.2(2015)
This study deals with treatment-dependent differences in sensitivity of Antarctic filamentous alga Zygnema sp. to photoinhibition. Zygnema sp. (strain EEL201) was collected at the James Ross Island, Antarctica (57° 52´ 57´´ W, 63° 48´ 02´´ S). In a laboratory, the alga was cultivated on agar first and then innoculated to liquid medium. They were exposed to a short-term (30 min.) high light (HL) treatments. Particular treatments comprised 600, 1 400 and 2 100 and 3 500 μmol m-2 s-1 of photosynthetically active radiation (PAR). Photosynthetic efficiency of Zygnema sp. in individual HL treatments was monitored by chlorophyll fluorescence parameters, potential (FV/FM) and actual (FPSII) quantum yield of photochemical processes in photosystem II in particular. Zygnema sp. showed a high resistance to HL since it both chlorophyll fluorescence parameters recovered to about 70% of initial values after 4 h in dark. Chlorophyll fluorescence measured immediately after particular treatment, showed HL-dependent decrease in absolute values of chlorophyll fluorescence signal and consequent uncompleted recovery as well. Quenching of F0, an indicator of changes in light-harvesting complexes of photosystem II, did not show dose-dependent response, however, general trend was a decrease found immediately HL treatment with consequent uncompleated recovery. In general, Zygnema sp. exhibited high resistance to PAR doses that the species can whitness in the field during austral summer. Thus the species could be considered highly adapted for high light and has effective mechanisms to cope with photoinhibition. Involvement of particular photoprotective mechanism, their activation and share in natural environment is a topic for future studies.
Zygnema sp.; high light treatment; chlorophyll fluorescence; quantum yield; potential yield
Adams III, W. W., Zarter, C. R., Mueh, K. E., Amiard, V. and Demmig-Adams, B. (2006): Energy dissipation and photoinhibition: A continuum of photoprotection. In: B. Demmig-Adams, William W. Adams III and A. K. Mattoo (eds): Photoprotection, photoinhibition, gene regulation, and environment. Springer, The Netherlands, pp. 49-64.
Aigner, S., Remias, D., Karsten, U. and Holzinger, A. (2013): Unusual phenolic compounds contribute to ecophysiological performance in the purple-colored green alga Zygogonium ericetorum (Zygnematophyceae, Streptophyta) from a high-alpine habitat. Journal of Phycology, 49: 648-660.
Anderson, B., Barber, J. (1996): Mechanism of photodamage and protein degradation during photoinhibition of photosystem II. In: Baker N. R. (ed.): Photosynthesis And The Enviroment. Kluwer Academic Publishers, Dordrecht, Netherlands, pp. 101-121.
Barták, M., Vráblíková, H. and Hájek, J. (2003): Sensitivity of photosystem 2 of antarctic lichens to high irradiance stress: fluorometric study of fruticose (Usnea antarctica) and foliose (Umbilicaria decussata) species. Photosynthetica, 41: 497-504.
Barták, M., Vráblíková-Cempírková, H., Štepigová, J., Hájek, J., Váczi, P. and Večeřová, K. (2008): Duration of irradiation rather than quantity and frequency of high irradiance inhibits photosynthetic processes in the lichen Lasallia pustulata. Photosynthetica, 46:161-169.
Becker, B., Marin, B. (2009): Streptophyte algae and the origin of embryophytes. Annals of Botany, 103: 999-1004.
Bilger, W., Schreiber, U. (1986): Energy-dependent quenching of dark-level chlorophyll fluorescence in intact leaves. Photosynthesis Research, 10: 303-308.
Choi, J-I., Yoon, M., Lim, S., Kim, G. H. and Park, H. (2014): Effect of gamma irradiation on physiological and proteomic changes of Arctic Zygnema sp. (Chlorophyta, Zygnematales). Phycologia, 54: 333-341.
Demmig-Adams, B., Adams III, W. W. (2000): Harvesting Sunlight Safely. Nature, 403: 371-374.
Elster, J., Benson, E. E. (2004): Life in the polar environment with a focus on algae and cyanobacteria. In: B. Fuller, N. Lane, E. Benson (eds.): Life in the Frozen State, Taylor and Francis, London, pp. 109-150.
Elster, J., Degma, P., Kováčik, L’., Valentová, L., Šrámková, K. and Batista Pereira, A. (2008): Freezing and desiccation injury resistance in the filamentous green alga Klebsormidium from the Antarctic, Arctic and Slovakia. Biologia, 63: 843-851.
Erikson, E., Wakao. S. and Niyogi, K. K. (2015): Light stress and photoprotection in Chlamydomonas reinhardtii. The Plant Journal, 82: 449-465.
Genkel, P. A., Pronina, N. D. (1979): Ecology of Zygnema stellinum Vauch. during desiccation of a shallow body of water. Biology bulletin of the Academy of Sciences of the USSR, 6: 504-509.
Germ, M., Kreft, I. and Gaberščik, A. (2009) UV-B radiation and selenium affected energy availability in green alga Zygnema. Biologia, 64: 676-679.
Graham, L.E., Arancibia-Avila, P., Taylor, W.A., Strother, P. K. and Cook, M. E. (2012): Aeroterrestrial Coleochaete (Streptophyta, Coleochaetales) models early plant adaptation to land. American Journal of Botany, 99: 130-144.
Hawes, I. (1990): Effects of freezing and thawing on a species of Zygnema (Chlorophyta) from the Antarctic. Phycologia, 29: 326-331.
Holzinger, A., Roleda, M. Y. and Lütz, C. (2009): The vegetative arctic freshwater green alga Zygnema is insensitive to experimental UV exposure. Micron, 40: 831-838.
Holzinger, A., Tschaikner, A. and Remias, D. (2010): Cytoarchitecture of the desiccation-tolerant green alga Zygogonium ericetorum. Protoplasma, 243: 15-24.
Jensen, M., Feige, G. B. (1991): Quantum efficiency and chlorophyll fluorescence in the lichens Hypogymnia psysodes and Parmelia sulcata. Lichen Biology, 11: 2-3.
Kadlubowska, J. Z. (1984): Conjugatophyceae I: Chlorophyta VIII: Zygnemales. In: H. Ettl, J. Gerloff, H. Heynig, D. Mollenhauer (eds.): Süsswasserflora von Mitteleuropa, Vol. 16, pp. 1-532.
Kang, S. H., Joo, H. M., Park, S., Jung, W., Hong, S. S., Seo, K-W., Jeon, M. S., Choi, H-G. and Kim, H. J. (2007): Cryobiological perspectives on the cold adaptation of polar organisms. Ocean Polar Research, 29: 263-271.
Kaplan, F., Lewis, L. A., Herburger, K. and Holzinger, A. (2013): Osmotic stress in Arctic and Antarctic strains of the green alga Zygnema (Zygnematales, Streptophyta): Effects on photosynthesis and ultrastructure. Micron, 44: 317-330.
Kappen, L., Schroeter, B., Green, T. G. A. and Seppelt, R. D. (1998): Chlorophyll a fluorescence and CO2 exchange of Umbilicaria aprina under extreme light stress in the cold. Oecologia, 113: 325-331.
Karsten, U., Holzinger, A. (2012): Light, temperature, and desiccation effects on photosynthetic activity, and drought-induced ultrastructural changes in the green alga Klebsormidium dissectum (Streptophyta) from a high alpine soil crust. Microbial ecology, 63: 51-63.
Kim, G. H, Klochkova, T. A. and Kang, S. H. (2008): Notes on freshwater and terrestrial algae from Ny-Alesund, Svalbard (high Arctic sea area). Journal of Environmental Biology, 29: 485-491.
Komárek, O., Felcmanová, K., Šetlíková, E., Kotabová, E., Trtílek, M. and Prášil, O. (2010): Microscopic measurements of the chlorophyll a fluorescence kinetics. In: D. J. Suggett, M. Borowitzka, O. Prášil (eds.): Chlorophyll a fluorescence in aquatic sciences: Methods and applications, developments in applied phycology. pp. 91-101.
Lovelock, C. E., Jackson, A. E., Melick, D. R. and Seppelt, R.D. (1995): Reversible photoinhibition in antarctic moss during freezing and thawing. Plant Physiology, 109: 955- 961.
Masojídek, J., Pechar, L., Koblížek, M., Adamec, L. and Komenda, J. (2001): Affinity of surface phytoplankton populations to high irradiance in hypertrophic fish ponds: implications of the competition between chlorococcal algae and cyanobacteria. Nova Hedwigia, 123: 255-273.
Mayba, N., Beckett, R. B., Csintalan, Z. and Tuba, Z. (2001): ABA increases the desiccation tolerance of photosynthesis in the afromontane understorey moss Atrichum androgynum. Annals of Botany, 88: 1093-1100.
McLean, R. J, Pessoney, G. F. (1971): Formation and resistance of akinetes of Zygnema. In: B. C. Parker, R. M. Brown Jr. (eds.): Contributions in Phycology, pp. 145-152.
Očenášová, P., Barták, M. and Hájek, J. (2014): Photoinhibition of photosynthesis in Antarctic lichen Usnea antarctica. II. Analysis of non-photochemical quenching mechanisms activated by low to medium light doses. Czech Polar Reports, 4: 90-99.
Orosa, M., Torres, E., Fidalgo, P. and Abalde, J. (2000): Production and analysis of secondary carotenoids in green algae. Journal of Applied Phycology, 12: 553-556.
Pichrtová, M. (2014): Stress resistance of polar hydro-terrestrial algae Zygnema spp. (Zygnematophyceae, Streptophyta). Ph.D. Thesis, Charles University, Prague, 146 p.
Pichrtová, M., Remias, D., Lewis, L. A. and Holzinger, A. (2013): Changes in phenolic compounds and cellular ultrastructure of arctic and antarctic strains of Zygnema (Zygnematophyceae, Streptophyta) after exposure to experimentally enhanced uv to PAR ratio. Microbial Ecology, 65: 68-83.
Pichrtová, M., Hájek, T. and Elster, J. (2014): Osmotic stress and recovery in field populations of Zygnema sp. (Zygnematophyceae, Streptophyta) on Svalbard (High Arctic) subjected to natural desiccation. FEMS microbiology ecology, 89: 270-280.
Poulíčková, A., Žižka, Z., Hasler, P. and Benada, O. (2007): Zygnematalean zygospores: morphological features and use in species identification. Folia microbiologica, 52: 135-145.
Rindi, F. (2007): Diversity, distribution and ecology of green algae and cyanobacteria in urban habitats. In: J. Seckbach (ed.): Algae and Cyanobacteria in Extreme Environments. Springer, Berlin, pp. 619-638.
Roháček, K. (2002): Chlorophyll fluorescence parameters: The definitions, photosynthetic meaning, and mutual relationships. Photosynthetica, 40: 13-29.
Stancheva, R., Sheath, R. G. and Hall, J. D. (2012): Systematics of the genus Zygnema (Zygnematophyceae, Charophyta) from Californian watersheds. Journal of Phycology, 48: 409-422.
Stamenkovic, M., Hanelt, D. (2013): Protection Strategies of Cosmarium strains (Zygnematophyceae, Streptophyta) Isolated from Various Geographic Regions Against Excessive Photosynthetically Active Radiation. Photochemistry and Photobiology, 89: 900-910.,
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